Isolation and Characterization of Biosurfactant Producing Pseudomonas aeruginosa YLA03 and its Diesel Degradation Potentials

This work was aimed at isolating and characterising biosurfactant producing bacteria strain from diesel oil contamination sites. The potential of the to degrade n-alkanes further studied. Soil samples were collected rich areas, identified strains grown on minimal media that contained as only carbon source. Enrichment culture technique used identify bacteria. Seventeen based their capacity use Other qualitative screening methods employed determine produce biosurfactants. Isolate A03 candidate shows positive signs for drop collapse, foaming, haemolytic, displacement emulsification tests. effect various parameters (incubation time, concentration diesel, nitrogen source, pH temperature) biodegradation evaluated. Optimum 8.0 35° C, while incubation time maximum growth five (5) days. favourable 7.5% isolate. isolate has shown degradative ability towards dodecane, Tridecane, tetradecane but unable 2, 6, 10-trimethyl- dodecane. It degraded between 10.09% - 45.39% individual alkanes. exhibited degrading alkanes pseudomonas aeruginosa A3 (Accession Number: MZ027605) using 16S rRNA. Sequencing.